Figure 8. The selective VRAC blocker DCPIB potently inhibits swelling-activated d-[³H]aspartate release and Cl⁻ currents.

A, effect of 20 μm DCPIB on swelling-activated d-[³H]aspartate release in the presence or absence of 20 μm ATP. Cells were exposed to hypoosmotic medium for 10 min, as indicated, in the presence (□, ○) or absence (▪, •) of DCPIB, which was given 10 min before and during application of hypoosmotic medium. ATP was present in hypoosmotic medium only (○, •). Data are means ± s.e.m. of 5 experiments. ***P < 0.001, DCPIB versus control; †††P < 0.001; ATP versus ATP plus DCPIB, repeated measures ANOVA. B, effect of 20 μm DCPIB on swelling-activated Cl⁻ currents. Representative of 6 electrophysiological recordings.