Fig. 3.

Voltage-dependent block of liposome reconstituted recombinant NR1a + NR2A receptor proteins by internal Mg²⁺. Membrane currents were obtained in response to voltage ramps from −100 mV to +100 mV in the absence (A) and presence (B) of 5 mM Mg²⁺ in the bath solution. Membrane currents were recorded in the inside-out recording configuration. The external pipette solution contained 10 μM glutamate, 100 μM glycine, 200 mM KCl, and 5 mM Hepes (pH 7.3). The internal bath solution facing the inside-out membrane contained 200 mM KCl, 5 mM MgCl₂, and 5 mM Hepes-KOH (pH 7.3) (n = 5 patches).