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illustrates controls for the detection of leptin mRNA. Figure 1a is a field from a male rat pituitary culture showing label for leptin mRNA with 100 ng/ml biotinylated antisense oligonucleotide probe complementary to leptin mRNA. Label is in irregular dense black patches in the cells (arrows). Figure 1b shows a field treated with 100 ng/ml biotinylated sense oligonucleotide leptin mRNA probe. No labeling is evident in this field. Bar=15 μm.
