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Cells from diestrous, proestrous AM and pregnant females were treated for 1 h with 1 nM GnRH. Media were assayed by EIA for leptin. Basal secretion from pregnant rat cultures is higher than that from diestrous rat cultures (open star). GnRH treatment stimulated higher media levels in all experimental groups (closed star). GnRH treated cultures from pregnant rats have higher media levels than all other groups. See text for statistics.
