A. RT-PCR carried out on Strongylocentrotus purpuratus cDNA prepared from gastrula stage embryos. B. Phylogenetic analysis of the sea urchin Wnt family. Confidence values were calculated using neighbor joining (green), maximum parsimony (blue), maximum likelihood (red star) and Bayesian (black) methods. The outgroup is defined by Wnt11 proteins. Accession numbers of all the proteins sequences used to elaborate the tree are indicated in Tables S1 and S2. Hs, Homo sapiens; Nv, Nematostella vectensis; Hv, Hydra vulgaris; Pd, Platynereis dumerilii; Pv, Patella vulgata; Sp, Strongylocentrotus purpuratus.