Figure 3-6921.

Up-regulated p75NTR expression is attributed to GAP43-positive neural processes. In dual immunofluorescence experiments, p75NTR expression in the peripheral processes (A, PP) overlapped with the expression of a marker for neural processes, GAP43 (B, PP), as shown here after 9 weeks of deafness, indicating that the elevation of p75NTR in the osseous spiral lamina is predominantly restricted to peripheral processes. The GAP43 antibody identified a ∼43-kd fragment (C, left lane) in immunoblots of hippocampal proteins, which was abolished in neutralization experiment with the antigenic peptide (C, right lane). C: Coomassie-stained gel demonstrated analysis of comparable amounts of proteins. Scale bars= 20 μm. Original magnification, ×40.