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. Author manuscript; available in PMC: 2007 Jan 23.
Published in final edited form as: Cancer Res. 2006 Sep 1;66(17):8581–8589. doi: 10.1158/0008-5472.CAN-06-0975

Figure 3.

Figure 3

JAB1 regulates p27 levels in pancreatic cancer cells. A, degradation of p27 was inhibited in Mia PaCa-2 and Panc-28 cells treated with proteasome inhibitors LLnL (35 μmol/L)and MG132 (30 μmol/L)but not in cells treated with LLM (25 μmol/L)or control DMSO. B, inducible expression of Ad-Myc-JAB1 down-regulated endogenous p27 levels in three pancreatic cancer cell lines as indicated. Lysates were prepared from Ad-Myc-JAB1-infected cells in the absence (-)or presence (+)of doxycycline (Dox)and proteins were immunoblotted with anti-Myc and anti-p27 antibodies. Anti-h-actin antibodies were used as a loading control. C, down-regulation of p27 by Ad-Myc-JAB1 is sensitive to 26S proteasome inhibitors. Panc-28 cells were transduced with the Ad-Myc-JAB1 virus and, 32 hours postinfection, 26S proteasome inhibitors LLnL and MG132 and DMSO as control were added for 16 hours. At 48 hours postinfection, whole-cell lysates were prepared and proteins analyzed by immunoblotting.