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. Author manuscript; available in PMC: 2007 Jan 23.
Published in final edited form as: Cancer Res. 2006 Sep 1;66(17):8581–8589. doi: 10.1158/0008-5472.CAN-06-0975

Figure 6.

Figure 6

Down-regulation of p27 by JAB1 in Skp2 knockdown Panc-28 cells. A, Skp2 siRNA induced p27 accumulation. Panc-28 cells were transfected with 50 nmol/L (+)and 100 nmol/L (++)of siSkp2 and with 100 nmol/L (++)of scrambled sequence as a control siRNA. Western blot analysis of Skp2 and p27 was done 48 hours after transfection. Anti-β-actin antibodies were used as loading controls. B, effect of Skp2 depletion on JAB1-mediated p27 degradation. Panc-28 cells were transfected with siSkp2 as described above. Cells were then transduced with Ad-Myc-JAB1 virus and subjected to Western blot analysis with the antibodies indicated on the left.