FIGURE 7.

The effects of anti-IFN-γ antibody (a, b) or exogenous IFN-γ (c, d) on serum BUN levels and intrarenal MRP1 expression. WT mice were treated with anti-IFN-γ antibody or recombinant IFN-γ, as described in Materials and Methods. a and c: Serum BUN levels were determined at 6 and 10 hours after NaAs challenge. All values represent means ± SEM (n = 8). *P < 0.05, control IgG versus anti-IFN-γ antibody, **P < 0.01; vehicle versus recombinant IFN-γ. b and d: Intrarenal MRP1 expression was examined at 10 hours after NaAs challenge, as described in Materials and Methods. Western blotting analysis using anti-β-actin antibody confirmed that an equal amount of protein was loaded onto each lane. Representative results from six independent experiments are shown here.