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. 2006 Oct;169(4):1390–1401. doi: 10.2353/ajpath.2006.060005

FIGURE 2.

FIGURE 2

MMP-3 is present in the nucleus of cultured cells. Immunodetection of MMP-3 in HepG2 cells and liver myofibroblasts. A: HepG2 nuclei were stained with DAPI. B: MMP-3 was detected using a polyclonal antibody. C: Merging of the signals confirms the mainly nuclear staining for MMP-3 in HepG2 cells. D: Myofibroblast nuclei were stained with DAPI. E: Immunofluorescent detection of MMP-3 in myofibroblasts with a monoclonal antibody directed against the proximal part of the hemopexin-like domain of MMP-3 (Santa Cruz 21732). F: Merging of the signals confirms the combined nuclear and cytoplasmic staining for MMP-3 in myofibroblasts. G–I: Double immunolabeling of myofibroblasts with the Santa Cruz 6839 antibody (G) showing only a cytoplasmic staining and with the polyclonal Chemicon antibody (H) showing a nuclear and cytoplasmic staining; I illustrates the nuclei stained with DAPI. J: Immunodetection of MMP-3 by confocal microscopy in myofibroblasts using the polyclonal antibody. K: Immunoelectron microscopy shows a strong nuclear labeling as well as some cytoplasmic staining for MMP-3 in a cultured myofibroblast.