FIGURE 1.

UV exposure results in the rapid activation of Erbb2 and is reduced by the inhibitor AG825. A: Mice were exposed to 10 kJ/m2 UV and epidermal protein extracts, obtained at the indicated times after UV, were immunoblotted as indicated (top panel), and relative Erbb2 phosphorylation was quantified by densitometry (bottom panel). Experiment shown in top panel is representative of three replicate experiments performed and quantified (bottom). B: Mice were topically treated with DMSO alone or AG825 in DMSO, exposed to 10 kJ/m2 UV, and euthanized at the indicated time points. A and B: Immunoblotting was performed with the indicated antibodies. Expression relative to actin as quantified by densitometry is shown. Arrow indicates the location of the EGFR family (including Erbb2), and graph indicates results of densitometry performed on the seven strongest bands detected, not including Erbb2. *P ≤ 0.05, significantly different versus time 0 using a Student’s t-test.