FIGURE 4.

Time course of wound healing and angiogenesis in mice transplanted with BM cells from EP3 receptor knockout mice. A lethal dose of radiation was given to WT mice, and BM cells either from WT mice or from EP3^−/− mice were injected into the tail vein under light ether anesthesia (WT→WT or EP3^−/−→WT). Surgical wounds were made on the backs of the mice, and wound closure was determined as described in Materials and Methods. a: Time course of wound closure in WT mice transplanted with BM cells either from EP3^−/− or from WT mice. Data are means ± SEM for the indicated number of mice. *P < 0.05 versus WT→WT mice (analysis of variance). b: The changes in the microvessel density in the wound granulation tissues in WT mice transplanted with BM cells either from EP3^−/− or from WT mice. After CD31 immunohistochemistry was photographed, the number of vessels in the observed area was counted and expressed per mm² observed. Data are means ± SEM for the indicated number of mice. P < 0.05 versus WT→WT (analysis of variance). c: Expressions of EP receptor subtypes in BM cells isolated from EP3^−/− mice and WT mice. RT-PCR was performed as described in the text.