Figure 6.

AT₁R blockade did not have renoprotective effects on immune-mediated glomerulonephritis in the AT₂R gene-deficient mice. a: The ERK pathway was significantly activated in the day 31 nephritic kidneys in the Agtr2⁺PC and Agtr2⁻PC groups. Although AT₁R blockade significantly suppressed ERK activation in the Agtr2⁺CGP group, it did not affect it in the Agtr2⁻CGP group. b: Urinary protein excretion was significantly increased in the Agtr2⁺PC and Agtr2⁻PC groups by day 56. AT₁R blockade significantly lowered urinary protein excretion in the Agtr2⁺CGP group, and it also reduced proteinuria in the Agtr2⁻CGP group. ci: The day 56 kidney tissues in the Agtr2⁻NC group. cii: The Agtr2⁻PC group. ciii: The Agtr2⁻CGP group. civ: The Agtr2⁺NC group. cv: The Agtr2⁺PC group. cvi: The Agtr2⁺CGP group (Masson’s trichrome). d: Glomerulosclerosis (GS) and interstitial fibrosis (IF) in the day 56 nephritic kidneys from each group were quantitated as described in Materials and Methods (versus NC, *P < 0.05; versus PC, ^#P < 0.05). The data in a were obtained from three independent experiments. Original magnifications, ×200.