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. 2006 Nov;169(5):1833–1842. doi: 10.2353/ajpath.2006.060234

Figure 5.

Figure 5

Effect of phosphatidylinositol 3-kinase/Akt signaling on Fas-mediated apoptosis in cholangiocarcinoma cells. Fas-sensitive (A, n = 6) and Fas-resistant (B, n = 3) cells were preincubated with inhibitors of PI 3-kinase/Akt signaling: AKTI (500 nmol/L), LY294002 (50 μmol/L), and Wortmannin (25 μmol/L) for 30 minutes and subsequently exposed to CH-11 (500 ng/ml) for 24 hours (*P < 0.05 for each inhibitor compared with CH-11 alone). C: Fas-sensitive cells were exposed to AKTI, Wortmannin, and LY294002 at the concentrations indicated for 30 minutes and subsequently exposed to CH-11 (500 ng/ml) for 24 hours (n = 3, *P < 0.05 compared with control). D: Fas-resistant cells were exposed to AKTI at 0 (control), 10, 40, 200, and 1000 nmol/L for 30 minutes and subsequently exposed to CH-11 (500 ng/ml) for 24 hours (n = 4, *P < 0.05 for AKTI + CH-11 compared with CH-11 only).