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. 2006 Nov;169(5):1833–1842. doi: 10.2353/ajpath.2006.060234

Figure 6.

Figure 6

Effect of adenovirus encoding dominant-negative (DN-AKT) and constitutively activated AKT (Ac-AKT) on Fas-mediated apoptosis. A: Cholangiocarcinoma cells at 50% confluence were exposed to control adenovirus or DN-AKT or Ac-AKT at 100 pfu/cell in serum-free DMEM/F12 media for 2 hours, and then DMEM containing 20% FBS was added to cells. Viral suspension was removed 16 hours after infection, and cells were grown in fresh growth media for 24 hours and subsequentially exposed to CH-11 (500 ng/ml) for 24 hours. DN-Akt adenovirus promoted, whereas constitutively activated Akt adenovirus protected, Fas-mediated apoptosis (n = 3, *P < 0.05). B: Western blot analyses of the expression of pAKT, p55FLIP, p43FLIP, and pIκB in cells with adenovirus-mediated altered expression of AKT. Representative blots of three independent experiments are shown. C: The density of each band was normalized to the density of GAPDH, and comparison of the mean densities of each protein in Ac-AKT or DN-Akt cells compared with the control cells was calculated as fold changes (n = 3, *P < 0.05).