Table 1.
Group | HT1080 cells, stably transfected | Number of animals | Doxycycline treatment |
---|---|---|---|
1 | Empty plasmid | 8 | − |
2 | Empty plasmid | 8 | + |
3 | CD97 (EGF 1,2,5) | 8 | − |
4 | CD97 (EGF 1,2,5) | 8 | + |
5 | CD97 (EGF 1,2,5/TM1) | 8 | − |
6 | CD97 (EGF 1,2,5/TM1) | 8 | + |
7 | CD97 (EGF 1-5) | 8 | − |
8 | CD97 (EGF 1-5) | 8 | + |
All animals underwent a subcutaneous injection with different tet-off HT1080 stably transfected clones. Every second animal group received doxycycline every third day to maintain down-regulation of CD97.