Figure 6.

Elevated numbers of IFN-γ-producing cells and NOS-2 production are associated with exacerbated liver damage in CD4⁺ T-cell-specific IL-4Rα-deficient mice. ELISPOT analysis of IFN-γ-producing (A) or IL-4-producing (B) cells from granuloma cell suspensions isolated from the liver of S. mansoni-infected mice. Experiment performed three times with similar results. C: mRNA levels of NOS-2 in liver tissue were determined by real-time PCR, normalized to β-2 microglobulin and expressed as fold increase over noninfected WT mice. Each symbol represents an individual mouse liver sample. Experiment performed three times with similar results. D: Serum levels of aspartate transaminase (AST), an indicator of hepatocellular damage expressed as mean ± SD of five individual samples per group. Results are representative of three experiments.