Table 2.
List of 10 CGIs Differentially Methylated in the Initial Samples of IM
| DNA fragments isolated by MS-RDA
|
Genes in the downstream
|
|||||
|---|---|---|---|---|---|---|
| Length (bp) | Accession no. | From | To | Chromosome | Gene symbol | Full names or alternative names |
| MS-RDA with HpaII | ||||||
| 227 | AC003682 | 72722 | 72845 | 19q13 | ZIK1 | Zinc finger protein-interacting with K protein 1 |
| 254 | AC108724 | 85358 | 85612 | 3p14 | LOC285300 | |
| 204 | AC064848 | 16801 | 17005 | 2p12 | CD8A | CD8 antigen, a polypeptide (p32) |
| MS-RDA with SacII | ||||||
| 405 | AC079140 | 120795 | 121200 | 4p16 | ZNF141 | Zinc finger protein 141 (clone pHZ-44) |
| 394 | AC005184 | 22438 | 22832 | Xp22 | KAL1 | Kallmann syndrome 1 |
| 374 | AE014303 | 198013 | 198387 | 13q34 | FGF14 | Fibroblast growth factor 14 |
| 382 | AL079303 | 21708 | 22090 | 14q13 | NKX2-8 | NK2 transcription factor-related, locus 8 |
| 384 | AL118557 | 77025 | 77409 | 14q21 | NID2 | Nidogen 2 (osteonidogen) |
| 346 | AL121904 | 65293 | 65639 | 20p11 | LOC284801 | |
| 190 | AC067859 | 139250 | 139440 | 2q31 | RAX | Retina and anterior neural-fold homeobox |
After analysis of 190 clones isolated by MS-RDA, 30 fragments were found to be derived from putative promoter CGIs. MSP analysis showed that these 10 CGIs were methylated in the samples with IM used for MS-RDA.