Figure 10.

Scrambled LfcinB does not compete with bFGF and VEGF₁₆₅ for binding sites on HUVECs. a: HUVEC monolayers were exposed to biotinylated bFGF (10 ng/ml) or biotinylated VEGF₁₆₅ (100 ng/ml) in the absence or presence of LfcinB or scrambled LfcinB (both at 50 μg/ml) for 2 hours. HUVEC monolayers were then washed and incubated for 2 hours with streptavidin-HRP. After additional washes, 1,2-phenylenediamine substrate was added, and absorbance was measured at 492 nm. Data are shown as mean absorbance ± SD of quadruplicate determinations. Background absorbance was 0.038 ± 0.001. Statistical significance relative to the medium control was determined by the Tukey-Kramer multiple comparisons test; *P < 0.05, **P < 0.01. b: HUVEC monolayers were exposed to biotinylated LfcinB or biotinylated scrambled LfcinB (both at 50 μg/ml) in the absence or presence of bFGF (10 ng/ml) or VEGF₁₆₅ (100 ng/ml) for 2 hours. HUVEC monolayers were then washed and incubated for 2 hours with streptavidin-HRP. After additional washes, 1,2-phenylenediamine substrate was added, and absorbance was measured at 492 nm. Data are shown as mean absorbance ± SD of quadruplicate determinations. Background absorbance was 0.063 ± 0.001. Statistical significance relative to the medium control was determined by the Tukey-Kramer multiple comparisons test; *P < 0.001.