Figure 9.

The HUVEC-binding capacity of LfcinB is superior to that of scrambled LfcinB or bovine lactoferrin. HUVEC monolayers were exposed to the indicated concentrations (63 and 15 μmol/L correspond to 200 and 50 μg/ml LfcinB, respectively) of biotinylated LfcinB, biotinylated scrambled LfcinB, or biotinylated lactoferrin for 2 hours. HUVEC monolayers were then washed and incubated for 2 hours with streptavidin-HRP. After additional washes, 1,2-phenylenediamine substrate was added, and absorbance was measured at 492 nm. Data are shown as mean absorbance ± SD of quadruplicate determinations. Background absorbance was 0.05 ± 0.0005. Statistical significance relative to the LfcinB control was determined by the Tukey-Kramer multiple comparisons test; *P < 0.001, **P < 0.01.