. 2000 Feb 7;2(2):154–164. doi: 10.1186/ar80

Table 2.

Frequency of, and tumour necrosis factor secretion by, Synovial T cells specific for cytomegalovirus

		TNF response (pg/ml)

Patient	HLA-A2-NLV⁺/CD8⁺ T cells (%)	10⁴	10⁵
RA3	0.2	3	45
RA11	2.3	21	102
RA14	0.5	10	61
RA15	13.9	60	70

Tumour necrosis factor (TNF) response was estimated by titrating the mononuclear cells after short-term incubation with COS cells transfected with human leucocyte antigen (HLA)-A2 and pp65 complementary DNA, as described by Scotet et al [16].