Figure 6.

Systemic delivery of Ad-mIL-4. Adenoviruses were injected into the tail vein 24 h after lipopolysaccharide treatment. (a) Arthritis index. Subsequently, severity of disease was assessed every other day using an established macroscopic scoring system ranging from 0 to 4: 0, normal; 1, detectable arthritis with erythma; 2, significant swelling and redness; 3, severe swelling and redness from joint to digit; and 4, maximal swelling with ankylosis or necrosis. +The average macroscopic score was expressed as a cumulative value for all paws, with a maximum possible score of 16 per mouse (n =10). ^*P <0.001;^**P <0.0005. (b) Paw swelling. The thickness of each paw was also evaluated using a spring-load caliper. The paw swelling for each mouse was calculated by adding the four thicknesses of the individual paws. ^*P <0.05; ^**P <0.001. (c) Number of arthritis paws. The total number of arthritic paws were determined for each experimental group (maximum 40 per group). ^*P <0.001.