Table 3.
Relative differential expression of selected genes following amputation and electroporation as determined by real-time RT-PCR
| Day 1 | Day 3 | Day 5 | ||||
|---|---|---|---|---|---|---|
| Gene | Amp | Elect | Amp | Elect | Amp | Elect |
| MMP3/10a | 42.3 ± 13.0 | 117.5 ± 9.1 | 4.0 ± 1.8 | 46.3 ± 20.7 | 4.7 ± 1.2 | 1.9 ± 0.3 |
| 169e | 40.7 ± 8.6 | 30.1 ± 5.4 | 6.9 ± 1.4 | 8.6 ± 1.2 | 2.5 ± 0.5 | 8.3 ± 1.0 |
| Fc Fragment of IgG binding prot. | 29.2 ± 3.8 | 2.8 ± 0.8 | 34.1 ± 5.2 | 28.7 ± 4.9 | 6.3 ± 2.3 | 38.1 ± 17.3 |
| 223d | 28.4 ± 3.7 | 20.1 ± 2.2 | 10.6 ± 3.8 | 14.6 ± 1.4 | 3.8 ± 0.6 | 6.6 ± 0.7 |
| 151b | 8.6 ± 1.5 | 17.9 ± 2.5 | 6.7 ± 1.2 | 8.3 ± 1.8 | 7.6 ± 1.8 | 4.5 ± 0.4 |
| Galectin 9 | 15.6 ± 4.8 | 9.7 ± 1.9 | 16.5 ± 3.7 | 6.6 ± 2.8 | 32.0 ± 6.1 | 5.7 ± 1.4 |
| Elafin-like 1 | 12.1 ± 1.6 | 8.6 ± 1.2 | 13.6 ± 0.8 | 17.0 ± 1.5 | 1.2 ± 0.1 | 7.8 ± 0.8 |
| 157b | 4.7 ± 0.7 | 9.6 ± 1.0 | 9.1 ± 0.7 | 9.2 ± 1.0 | 3.1 ± 0.4 | 4.0 ± 0.4 |
| 125c | 5.8 ± 1.2 | 10.5 ± 1.1 | 9.1 ± 1.1 | 7.4 ± 0.9 | 5.0 ± 0.6 | 3.7 ± 0.5 |
| 4 Apple domain protein | 11.4 ± 1.6 | 6.2 ± 0.7 | 2.2 ± 0.1 | 2.1 ± 0.3 | 8.7 ± 0.9 | 1.0 ± 0.1 |
| Variable lymphocyte receptor A | 1.5 ± 0.2 | 3.1 ± 0.4 | 8.4 ± 0.6 | 5.9 ± 0.5 | 2.0 ± 0.3 | 4.8 ± 0.4 |
| 174a | 5.9 ± 1.2 | 13.7 ± 1.5 | 7.9 ± 0.9 | 14.5 ± 1.3 | 3.5 ± 0.4 | 6.3 ± 0.5 |
| 193c | 0.3 ± 0.0 | 2.4 ± 0.3 | 3.4 ± 0.6 | 5.4 ± 0.5 | 0.6 ± 0.1 | 3.9 ± 0.3 |
| Profilin 2 | 2.0 ± 0.3 | 2.6 ± 0.2 | 3.8 ± 0.1 | 2.0 ± 0.2 | 2.4 ± 0.2 | 1.9 ± 0.2 |
| 117g | 0.7 ± 0.1 | 2.5 ± 0.5 | 0.9 ± 0.2 | 0.7 ± 0.2 | 0.9 ± 0.1 | 0.4 ± 0.1 |
| FLN29-like gene | 1.8 ± 0.2 | 3.0 ± 0.2 | 0.7 ± 0.3 | 1.5 ± 0.3 | 1.7 ± 0.2 | 1.0 ± 0.2 |
| Activating transcription factor 4 | 2.7 ± 0.5 | 2.7 ± 0.3 | 1.8 ± 0.3 | 1.5 ± 0.2 | 2.0 ± 0.3 | 1.4 ± 0.1 |
| Ribophorin II | 2.9 ± 0.6 | 2.2 ± 0.2 | 1.7 ± 1.1 | 1.1 ± 0.3 | 2.8 ± 0.6 | 1.1 ± 0.5 |
| DEAD box polypeptide 1 | 0.8 ± 0.3 | 2.2 ± 0.3 | 0.2 ± 0.2 | 1.2 ± 0.4 | 1.1 ± 0.0 | 1.0 ± 0.1 |
| Ras-related protein Rab 11A | 1.9 ± 0.7 | 1.4 ± 0.4 | 2.1 ± 1.0 | 1.4 ± 1.0 | 1.5 ± 0.3 | 1.3 ± 1.0 |
| Inositol 1,3,4,5,6-pentakisphosphate 2-kinase | 1.0 ± 0.4 | 1.3 ± 0.4 | 1.0 ± 0.5 | 1.0 ± 0.2 | 0.7 ± 0.3 | 1.0 ± 0.2 |
| 113c | 1.0 ± 0.1 | 1.5 ± 0.5 | 1.0 ± 0.2 | 1.1 ± 0.4 | 0.7 ± 0.1 | 1.2 ± 0.2 |
| 105d | 0.7 ± 0.2 | 0.8 ± 0.4 | 1.3 ± 0.2 | 1.2 ± 0.6 | 0.7 ± 0.2 | 0.9 ± 0.2 |
| Ribosomal protein L27 | 0.3 ± 0.0 | 0.7 ± 0.1 | 0.7 ± 0.1 | 0.7 ± 0.1 | 0.3 ± 0.0 | 0.6 ± 0.1 |
Numbers represent x-fold upregulation following amputation (Amp) or electroporation (Elect). Standard deviations for each of these values are shown. Values were normalized to the histone acetyltransferase 1 gene.