Figure 6.

Novel exons are supported by peptide identifications and by sequence homology. Above is a multiple alignment for hypothetical protein sequences from chimp (gi:55639283), rat (gi:62531299), and human (genome translation, similar to PGAM5 gi:20070384). Introns are indicated by colons. The peptides identified from mass spectra are indicated below the protein sequence. The novel 3′ exon is supported by three peptide identifications, as well as >95% amino acid sequence conservation across species.