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RNA sample purity. (A) The secondary structure of the TAR RNA is shown. (B) Imino proton region of 1D 1H NMR spectra of HIV TAR RNA purified by size-exclusion chromatography. Assignments are indicated numerically above the peaks. (C) Denaturing 10% TBE gel of the NMR sample used in B, stained with toluidine blue.
