FIGURE 1.

Editing in RNA substrates with various lengths of 5′ (A) and 3′ (B) sequence. Gray boxes represent the substrates containing psbE sequence from Arabidopsis (A) and tobacco (B) with the number of nucleotides 5′ and 3′ indicated. Electrophoretograms of the poisoned primer extension (PPE) reactions used to assay editing efficiencies are shown below the substrate diagrams, with the % editing calculated as described previously (Peeters and Hanson 2002). (Lane O) PPE without template, indicating the size of the labeled oligo; (lane UE) PPE of substrate incubated without competent editing extract. All other lanes are labeled according to the length of sequence 5′ (A)and 3′ (B).