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Editing in C214 and C215 in substrates with deletions or insertions compared with wild-type tobacco sequence. (A) Alignment of mutant substrates constructed to examine spacing constraints for editing. Bolded characters represent deleted or inserted nucleotides. Gray bars depict the percentage of editing of mutant substrates at the normal editing site C214 (B) and the 3′ adjacent nucleotide C215 (C). (B,C) Error bars are calculated from duplicate reactions as 1 SD from the mean.
