Table 1.
Sample ID | Subtype1 | CH3 | NF2 gene mutations | DNA copy number profiles4 | ||||
Position | Mutation2 | Assumed effect3 | Chromosome 22 | Chromosome 1 | Loss of non-22 genes5 | |||
M26 | A | Ex 2 | 227_240del | Lys76fs | Monosomy | Monosomy | D1, S1A, S1B | |
M71 | A | Ex8 | 810+2T>C | Abn. Spl. | Monosomy | 1p, 120.8 Mb TD | D1, P2A, S1A, S1B | |
M38 | A | Ex6 | 573del; 575_581del | Trp191fs | Monosomy | 1p 107.9 Mb TD | P1B, P2A, S1A, S1B | |
M43 | A | Ex12 | 1185del | Lys396fs | Monosomy | 1p 120.3 Mb TD, 4.4 Mb A | ||
M46 | A | Ex8 | 810+2T>C | Abn. Spl. | NAD | NAD | ||
M33 | A | NAD | 30.6 Mb TD [AC009516-AC002055] | 1p, 90.2 Mb TD, 45.8 Mb ID | ||||
M9 | A | NAD | NAD | NAD | ||||
M20 | A | NAD | NAD | NAD | ||||
M13 | F | Ex 2 | 240+2T>C | Abn. Spl. | Monosomy | 1p, 142.9 Mb TD | P2A, S1A, S1B | |
M88 | F | Ex4 | 364-1G>A | Abn. Spl. | Monosomy | 1p 84.1 Mb TD; 1q 4.6, 7.1 Mb IDs, 12.5 Mb TD | P2A | |
M3 | F | Ex 2 | 240+2T>C | Abn. Spl. | Monosomy | 1p 119.8 Mb TD | ||
M14 | F | Ex3 | 249del | His84fs | Monosomy | 1p 120.2 Mb TD | ||
M15 | F | Ex8 | 683del | Lys228fs | Monosomy | 1p 116.0 Mb TD | ||
M39 | F | Ex8 | 810+1G>A | Abn. Spl. | Monosomy | NS | ||
M42 | F | Ex10 | 995del | Lys332fs | Monosomy | NS | ||
M70 | F | Ex6 | 599+1G>A | Abn. Spl. | Monosomy | NS | ||
M91 | F | Ex 2 | 154del | Arg52fs | Monosomy | NS | ||
M102 | F | Ex9 | 865A>T | Lys289X | Monosomy | NS | ||
M115 | F | Ex7 | 634C>T | Gln212X | NAD | 1p 119.6 Mb TD | ||
M646 | F | NAD | 30.4 Mb TD [AC008079-AC002055], NF2 biallelic del [AC005529] | 1p 98.8 Mb ID; 1q 24.2 Mb ID | P1B, P2A | |||
M66 | F | NAD | Monosomy | 1p 118.1 Mb TD | P2A | |||
M117 | F | NAD | 0.55, 2.8, 4.5 Mb IDs [U07000-AP00034, AC005004-Z69907, AL022311-Z83851]; 5.9 Mb TD [Z93244-AC002055] | 1p 116.8 Mb TD, 3.6 Mb A | ||||
M32 | F | NAD | 5.3 Mb ID [AL022329-AL022336], 15.2 Mb TD [AL009049-AC002055] | NS | D1 | |||
M4 | F | NAD | Monosomy | NAD | ||||
M29 | F | NAD | Monosomy | NS | ||||
M41 | F | NAD | Monosomy | NS | ||||
M92 | F | NAD | Monosomy | NS | ||||
M114 | F | NAD | 18.0 Mb ID [AP000525-Z68324] | NS | ||||
M119 | F | NAD | NAD | NS | ||||
M10 | M | Ex13 | 1341-2A>G | Abn. Spl. | Monosomy | 1p 120.3 Mb TD | P2A | |
M23 | M | Ex3 | 241-2A>C | Abn. Spl. | Monosomy | 1p 120.9 Mb TD | P2A | |
M67 | M | Ex4 | 432C>A | Tyr144X | Monosomy | 1p 120.8 Mb TD | P2A | |
M98 | M | Ex14 | 1519_1520del | Phe507fs | Monosomy | NAD | D1, P2A, S1A, S1B | |
M57 | M | Ex 2 | 133_144 del | Asp45fs | Monosomy | NS | ||
M72 | M | Ex7 | 634C>T | Gln212X | Monosomy | NS | ||
M40 | M | NAD | Monosomy | 1p 164.9 Mb TD | P1B, P2A | |||
M76 | M | NAD | Monosomy | 1p 142.7 Mb TD | P2A | |||
M105 | M | NAD | Monosomy | 1p 116.3 Mb TD | P1B, P2A | |||
M348 | M | NAD | 20.0 Mb TD [AC004542-AC002055] | 1p 102.8 Mb ID | ||||
M44 | M | NAD | Monosomy | 1p 85.4 Mb TD | ||||
M101 | M | NAD | 25.1 Mb ID [AP000525-AL035450], 7.9 Mb TD [AL049758-AC002055] | Monosomy, 1p 2.1 Mb R | ||||
M30 | M | NAD | Monosomy | NAD | D1, P2A | |||
M112 | M | NAD | NAD | 1p 114.2 Mb TD | ||||
M2 | M | NAD | NAD | NAD | ||||
M5 | M | NAD | NAD | NAD | ||||
M77 | M | NAD | NAD | NAD | ||||
M82 | M | NAD | NAD | NAD | ||||
M45 | M | NAD | NAD | NS | ||||
M51 | M | NAD | NAD | NS | ||||
M54 | M | NAD | NAD | NS | ||||
M61 | M | NAD | NAD | NS | ||||
M63 | M | NAD | NAD | NS | ||||
M65 | M | NAD | NAD | NS | ||||
M69 | M | NAD | NAD | NS | ||||
M86 | M | NAD | NAD | NS | ||||
M95 | M | NAD | NAD | NS | ||||
M99 | M | NAD | NAD | NS | ||||
M107 | M | NAD | NAD | NS | ||||
M108 | M | NAD | NAD | NS | ||||
M111 | M | NAD | NAD | NS | ||||
M116 | M | NAD | NAD | NS | ||||
M120 | M | NAD | NAD | NS | ||||
M93 | P | Ex8 | 810G>T | Glu270Asp | Monosomy | 1p 114.3 Mb ID | P2A | |
M229 | P | Ex13, inter1 | 1390del; 28309611 C>A | Ala464fs, unknown | Monosomy | NS | ||
M62 | P | NAD | Monosomy | NS | ||||
M21 | P | NAD | NAD | NS | ||||
M60 | T | Ex14 | 1519_1528del | Phe507fs | Monosomy | 1p 118.2 Mb TD | P2A | |
M1 | T | Ex7 | 650del | Tyr217X | Monosomy | 1p 65.0 Mb TD | ||
M31 | T | Ex13 | 1325del | Ala451fs | Monosomy | 1p 53.2, 36.3 Mb IDs | ||
M58 | T | Ex 2 | 115-3G>C | Abn. Spl. | 30.1 Mb TD [AC007050-AC002055] | 1p 120.2 Mb TD | ||
M90 | T | Ex3 | 241-2A>G | Abn. Spl. | 20.1Mb ID [AP000525-AL079295], 13.4 Mb TD [AL049850-AC002055] | 1p 83.3 Mb TD | ||
M96 | T | Ex12 | 1175G>T | Glu392X | 2.0 Mb ID [AP000525-AC007666], 5.4 Mb A [AC006285-AC000102], 27.5 Mb TD [U07000-AC002055] | 1p 43.3 Mb TD, 1q 69.9 Mb TD | ||
M52 | T | Ex4 | 431_432insAA | Tyr144X | Monosomy | NS | P1B | |
M113 | T | Ex4 | 377del | Leu127X | Monosomy | NAD | D1, P1B, P2A, S1A, S1B | |
M18 | T | Ex12 | 1198C>T | Gln400X | Monosomy | NS | ||
M37 | T | Ex8 | 745_755del | Arg249fs | Monosomy | NS | ||
M48 | T | Ex 1 | 36_37del | Leu14fs | Monosomy | NS | ||
M75 | T | Ex8 | 750del | Thr251fs | Monosomy | NS | ||
M87 | T | Ex4 | 436G>A | Val146Ile | NAD | NS | ||
M56 | T | NAD | Monosomy | 1p 116.5 Mb TD | P2A | |||
M85 | T | NAD | Monosomy | 1p 108.2 Mb TD | P2A | |||
M109 | T | NAD | 5.7 Mb A [AC006548-AC007957], 30.6 Mb TD [AC009516-AC002055] | 1p 120.2 Mb TD | ||||
M27 | T | NAD | 15.5Mb ID [AP000525-AC005005], 14.1 Mb TD [AL049749-AC002055] | NS | ||||
M59 | T | NAD | Monosomy | NS | ||||
M68 | T | NAD | Monosomy | NS | ||||
M94 | T | NAD | Monosomy | NS | ||||
M97 | T | NAD | Monosomy | NS | ||||
M25 | T | NAD | NAD | 1p 98.7 Mb TD | ||||
M117 | T | NAD | NAD | 1p 36.1 Mb TD | ||||
M10410 | T | + | NAD | NAD | NS | |||
M8 | T | NAD | NAD | NS | ||||
M12 | T | NAD | NAD | NS | ||||
M17 | T | NAD | NAD | NS | ||||
M35 | T | NAD | NAD | NS | ||||
M55 | T | NAD | NAD | NS | ||||
M89 | T | NAD | NAD | NS | ||||
M103 | T | NAD | NAD | NS | ||||
M78 | U | Ex10 | 999+1G>T | Abn. Spl. | Monosomy | 1p 3.8 Mb TD | ||
M79 | U | NAD | NAD | 1p 39.1Mb ID, 1q 41.3Mb TD | ||||
M83 | U | NAD | NAD | NS |
1 Tumors are grouped according to histopathological subtypes. Abbreviations: A, Anaplastic; F, Fibroblastic; M, Meningothelial; P, Psammomatous; T, Transitional and U, Unclassified.
2 Position of the mutations according to numbering of the cDNA sequence, nucleotide number one is the A in the ATG translation initiation codon [GenBank:AF369658]. Abbreviations: substitution, >; deletion, del; insertion, ins; No Aberration Detected, NAD. Donor splice site mutations indicated by the last nucleotide of the preceding exon, a plus sign and position in the intron (e.g. 240+2T>C). Acceptor splice site mutations denoted by the first nucleotide of the following exon, a minus sign and position in the intron (e.g. 241-2A>C).
3 Amino acid number one is the translation initiator Methionine. Abbreviations: translation termination codon, X; frame shift, fs; abnormal splicing, Abn. Spl.
4 Data from copy number imbalances on chromosome 1 were previously published [34]. Abbreviations: Terminal Deletion, TD; Interstitial Deletion, ID; Amplification/gain, A; Not Studied, NS; No Aberration Detected, NAD; Retained segment R. Genbank accession numbers in squared brackets.
5 Loss of non-chromosome 22 clones spanning the DAL1, D1; PTCH2A, P2A; PTCH1B, P1B; SUFU1A, S1A; and SUFU1B, S1B genes.
6 M64 displays a homozygous deletion of the NF2 gene.
7 M11 demonstrates multiple deletions not affecting the NF2 gene.
8 M34 displays a terminal deletion not affecting the NF2 locus.
9 M22 presents two mutations in the NF2 locus, in exon 13 and in the CNG inter1. The position of the inter1 mutation is according to numbering on chromosome 22 (UCSC Homo sapiens Genome Browser, assembly July 2003). Ten bases on each side of the mutation: agcaaagagaCaaattaccca.
10 Methylation of a single CpG site in the intron 1 of the NF2 gene in ~40% of DNA molecules.