Figure 1.

Transgene construction and βgal staining of lung lobes. A. Transgene fragment for microinjection. The Cre cDNA and a Neo polyadenylylation signal were placed under the control of the mouse α-SMA promoter (-1070 to +2582, including the first exon and part of the first intron). B. Comparison of βgal staining in the bronchi of R26R and α-SMA-Cre/R26R mice. Positive βgal staining (blue color) is observed in the bronchi of α-SMA-Cre/R26R mice (a, 20× magnification), but not in R26R mice (b, 20× magnification).