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. 2007 Jan 26;3(1):e11. doi: 10.1371/journal.ppat.0030011

Figure 8. Activated B Cells Are Decreased in the Lungs and Spleens of IκBαM-Infected Mice.

Figure 8

Cells were prepared from the lungs and spleens harvested from four uninfected naïve mice or from four or five mice that were infected 16 and 42 d prior to harvest with 1,000 PFU of WT γHV68 or IκBαM.1. Cells from individual mice were surface stained with anti-CD19 conjugated to allophycocyanin and anti-CD69 conjugated to FITC and analyzed by flow cytometry.

(A) Flow cytometric dotplots of stained cells from the lungs (upper panel) and spleens (lower panel). Values shown in the upper right quadrant of each dotplot are the percentages of CD19+ cells that express the CD69 activation marker in a representative experimental sample.

(B) Each point in the scatterplot represents the percentage of CD19+CD69hi cells from the lung of a single mouse; the bar represents the mean percentage. The percentage of CD19+CD69hi cells in the lungs of mice infected with IκBαM.1 was significantly different from that of mice infected with WT γHV68; p = 0.0007 at 16 dpi.

(C) Each point in the scatterplot represents the percentage of CD19+CD69hi cells from the spleen of a single mouse; the bar represents the mean percentage. The percentage of CD19+CD69hi cells in the spleens of mice infected with IκBαM.1 was significantly different from that of mice infected with WT γHV68; p < 0.0001 at 16 dpi and p = 0.0042 at 42 dpi.