Skip to main content
. 2007 Jan 26;3(1):e11. doi: 10.1371/journal.ppat.0030011

Figure 9. Route- and Tissue-Dependent Requirement for NF-κB.

Figure 9

C57Bl6 mice were infected with 1,000 PFU by the intraperitoneal route of inoculation with the indicated viruses.

(A) On 4 and 9 dpi, spleens were harvested, disrupted, and titered on NIH 3T12 cells. The data are compiled from two (IκBαM.MR and WT) or four (IκBαM.1) experiments with four or five mice per group. Asterisks denote that the acute splenic titers for IκBαM.1 differed significantly from IκBαM.MR on day 4 (p = 0.0036) and from IκBαM.MR (p < 0.0001) and WT (p < 0.0001) on day 9, as determined by the Mann-Whitney nonparametric test.

(B) Frequency of PECs harboring viral genomes.

(C) Frequency of unsorted, bulk splenocytes harboring viral genome.

(D) Frequency of PECs reactivating virus.

(E) Frequency of splenocytes reactivating virus. For both limiting-dilution assays, curve fit lines were derived from nonlinear regression analysis, and symbols represent the mean percentage of wells positive for virus (viral DNA or cytopathic effect) ±SEM. The data shown represent three to five independent experiments with cells pooled from five mice per experimental group.