Fig. 1.

Schematic of the differentiation induction subtraction hybridization (DISH) approach. This procedure has been used to identify and clone genes displaying differential expression as a function of induction of irreversible growth arrest, terminal differentiation, and loss of tumorigenic potential in HO-1 human melanoma cells. Temporally spaced libraries are constructed from actively growing HO-1 cells and from HO-1 cells treated with combination of IFN-β plus MEZ. The actively growing HO-1 cDNA library is then subtracted from the IFN-β plus MEZ-treated HO-1 library resulting in a subtracted cDNA library enriched for differentially expressed genes that associate with a multitude of processes, some of which are indicated in this figure. Further details of the subtraction approach and its application with reverse Northern blotting of cDNAs and high throughput cDNA microarrays can be found in Jiang and Fisher (1993) and Huang et al. (1999a, 1999b) (reproduced, by permission of the publisher, from Fisher et al., 2003).