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. Author manuscript; available in PMC: 2007 Jan 25.
Published in final edited form as: Pharmacol Ther. 2006 Feb 7;111(3):596–628. doi: 10.1016/j.pharmthera.2005.11.005

Fig. 6.

Fig. 6

Inhibition of the p38 MAPK pathway protects FO-1 melanoma cells from Ad.mda-7-mediated cell death. (A) FO-1 cells were infected with either Ad.vec or Ad.mda-7 (100 pfu/cell) and treated with 1 μM SB203580 or infected with Ad.p38DN (an adenovirus expressing a dominant-negative p38 mutant gene) (100 pfu/cell). Cell viability was measured by MTT assay after 4 days. Cell viability of Ad.vec-treated cells was regarded as 1. *Significant differences from Ad.mda-7 (P<0.0001). (B) FO-1 cells were infected with either Ad.vec or Ad.mda-7 (100 pfu/cell) and treated with 1 μM SB203580 for 3 days. DNA was isolated from the cells and fragmentation was analyzed as described in Sarkar et al. (2002b). (C) FO-1 cells were infected with either Ad.vec or with Ad.mda-7 (100 pfu/cell) and treated with 1 μM SB203580 or infected with Ad.p38DN (100 pfu/cell). Percentage of apoptotic cells at days 1 and 3 after infection in each group were plotted (reproduced, by permission of the publisher, from Sarkar et al., 2002b).