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Fluorescence-based X-inactivation using PCR-based AR methylation assay. (a) An example of equivalent inactivation (51% versus 49%) of both upper (271 bp) and lower (260 bp) alleles. (b) An example of extremely skewed inactivation (10% versus 90%) of the upper (284 bp) and lower (271 bp) alleles. Mock-digested DNA (without HpaII) was used as control for peak intensities of both alleles.
