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. 2003 Oct;12(5):285–292. doi: 10.1080/0929350310001619744

T helper cell polarisation as a measure of the maturation of the immune response.

Scott B Cameron 1, Ellen H Stolte 1, Anthony W Chow 1, Huub F J Savelkoul 1
PMCID: PMC1781629  PMID: 14760935

Abstract

BACKGROUND: T helper cell polarisation is important under chronic immune stimulatory conditions and drives the type of the evolving immune response. Mice treated with superantigens in vivo display strong effects on Th subset differentiation. The aim of the study was to detect the intrinsic capacity of T cells to polarise under various ex vivo conditions. METHODS: Purified CD4+ T cells obtained from super-antigen-treated mice were cultured under Th polarising conditions in vitro. By combining intracellular cytokine staining and subsequent flow cytometric analysis with quantitative cytokine measurements in culture supernatants by enzyme-linked immunosorbent assay (ELISA), the differential Th polarising capacity of the treatment can be detected in a qualitative and quantitative manner. RESULTS AND CONCLUSIONS: BALB/c mice were shown to be biased to develop strong Th2 polarised immune responses using Th0 stimulation of purified CD4+ T cells from phosphate-buffered saline-treated mice. Nevertheless, our analysis methodology convincingly showed that even in these mice, Toxic Shock Syndrome Toxin-1 treatment in vivo resulted in a significantly stronger Th1 polarising effect than control treatment. Our results indicate that populations of Th cells can be assessed individually for their differential Th1 or Th2 maturation capacity in vivo by analysing robust in vitro polarisation cultures combined with intracellular cytokine staining and ELISA.

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Selected References

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  1. Abbas A. K., Murphy K. M., Sher A. Functional diversity of helper T lymphocytes. Nature. 1996 Oct 31;383(6603):787–793. doi: 10.1038/383787a0. [DOI] [PubMed] [Google Scholar]
  2. Abraham C., Miller J. Molecular mechanisms of IL-2 gene regulation following costimulation through LFA-1. J Immunol. 2001 Nov 1;167(9):5193–5201. doi: 10.4049/jimmunol.167.9.5193. [DOI] [PubMed] [Google Scholar]
  3. Cameron S. B., Nawijn M. C., Kum W. W., Savelkoul H. F., Chow A. W. Regulation of helper T cell responses to staphylococcal superantigens. Eur Cytokine Netw. 2001 Apr-Jun;12(2):210–222. [PubMed] [Google Scholar]
  4. Chtanova T., Kemp R. A., Sutherland A. P., Ronchese F., Mackay C. R. Gene microarrays reveal extensive differential gene expression in both CD4(+) and CD8(+) type 1 and type 2 T cells. J Immunol. 2001 Sep 15;167(6):3057–3063. doi: 10.4049/jimmunol.167.6.3057. [DOI] [PubMed] [Google Scholar]
  5. Cubillas-Tejeda A. C., Ruiz-Argüelles A., Bernal-Fernández G., Quiroz-Compeán L., López-Dávila A., Reynaga-Hernández E., González-Amaro R. Cytokine production and expression of leucocyte-differentiation antigens by human mononuclear cells in response to mycobacterium tuberculosis antigens. Scand J Immunol. 2003 Feb;57(2):115–124. doi: 10.1046/j.1365-3083.2003.01200.x. [DOI] [PubMed] [Google Scholar]
  6. Hodge Deborah L., Martinez Alfredo, Julias John G., Taylor Lynn S., Young Howard A. Regulation of nuclear gamma interferon gene expression by interleukin 12 (IL-12) and IL-2 represents a novel form of posttranscriptional control. Mol Cell Biol. 2002 Mar;22(6):1742–1753. doi: 10.1128/MCB.22.6.1742-1753.2002. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. House R. V. Theory and practice of cytokine assessment in immunotoxicology. Methods. 1999 Sep;19(1):17–27. doi: 10.1006/meth.1999.0823. [DOI] [PubMed] [Google Scholar]
  8. Infante-Duarte C., Kamradt T. Th1/Th2 balance in infection. Springer Semin Immunopathol. 1999;21(3):317–338. doi: 10.1007/BF00812260. [DOI] [PubMed] [Google Scholar]
  9. Kelso A., Groves P., Ramm L., Doyle A. G. Single-cell analysis by RT-PCR reveals differential expression of multiple type 1 and 2 cytokine genes among cells within polarized CD4+ T cell populations. Int Immunol. 1999 Apr;11(4):617–621. doi: 10.1093/intimm/11.4.617. [DOI] [PubMed] [Google Scholar]
  10. Misra N., Murtaza A., Walker B., Narayan N. P., Misra R. S., Ramesh V., Singh S., Colston M. J., Nath I. Cytokine profile of circulating T cells of leprosy patients reflects both indiscriminate and polarized T-helper subsets: T-helper phenotype is stable and uninfluenced by related antigens of Mycobacterium leprae. Immunology. 1995 Sep;86(1):97–103. [PMC free article] [PubMed] [Google Scholar]
  11. Sander B., Andersson J., Andersson U. Assessment of cytokines by immunofluorescence and the paraformaldehyde-saponin procedure. Immunol Rev. 1991 Feb;119:65–93. doi: 10.1111/j.1600-065x.1991.tb00578.x. [DOI] [PubMed] [Google Scholar]
  12. Schauer U., Jung T., Krug N., Frew A. Measurement of intracellular cytokines. Immunol Today. 1996 Jul;17(7):305–306. doi: 10.1016/0167-5699(96)30020-0. [DOI] [PubMed] [Google Scholar]
  13. Verhagen C. E., van der Pouw Kraan T. C., Buffing A. A., Chand M. A., Faber W. R., Aarden L. A., Das P. K. Type 1- and type 2-like lesional skin-derived Mycobacterium leprae-responsive T cell clones are characterized by coexpression of IFN-gamma/TNF-alpha and IL-4/IL-5/IL-13, respectively. J Immunol. 1998 Mar 1;160(5):2380–2387. [PubMed] [Google Scholar]
  14. van Zijverden M., van der Pijl A., Bol M., van Pinxteren F. A., de Haar C., Penninks A. H., van Loveren H., Pieters R. Diesel exhaust, carbon black, and silica particles display distinct Th1/Th2 modulating activity. Toxicol Appl Pharmacol. 2000 Oct 15;168(2):131–139. doi: 10.1006/taap.2000.9013. [DOI] [PubMed] [Google Scholar]

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