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. 2000 Jul 3;2(5):415–423. doi: 10.1186/ar120

Table 3.

Primers used for PCR amplification

Cytokine/chemokine receptor Primer
IL-2 5'-TAC AAG AAT CCC AAA CTC ACC AGG A
3'-GTC AGT GTT GAG ATG ATG CTT TGA CA
3' (nested)-TGG TTG CTG TCT CAT CAG CAT ATT CA
IL-4 5'-CTT TGC TGC CTC CAA GAA CAC AAC T
3'-TCT CAT GAT CGT CTT TAG CCT TTC CA
3' (nested)-TCC TTC ACA GGA CAG GAA TTC AAG C
IL-6 5'-GTT CCT GCA GAA AAA GGC AAA G
3'-CTG AGG TGC CCA TGC TAC ATT T
5' (nested)-CTA GAT GCA ATA ACC ACC CCT GA
IL-10 5'-TTA GGA AGA GAA ACC AGG GAG CC
3'-GCT GGC CAC AGC TTT CAA GAA T
5' (nested)-CAG GGA GCC CCT TTG ATG ATT A
IL-13 5'-ACC CAG GGA TGA CAT GTC CCT A
3'-CCC AAC GGT GAC AAA CAC ACT C
3' (nested)-AAG AAG TGT GCC TGA GCT GCT GT
IFN-γ 5'-AAG GCT TTA TCT CAG GGG CCA ACT
3'-TGG AAG CAC CAG GCA TGA AAT CTC
5' (nested)-CAA GAT CCC ATG GGT TGT GTG TTT
TNF-α 5'-GGC TCA GAC ATG TTT TCC GTG A
3'-CTC AGC AAT GAG TGA CAG TTG G
5' (nested)-CAA TAG GCT GTT CCC ATG TAG CC
LT-α 5'-CCT GAA CCA TCC CTG ATG TCT G
3'-AAA TAG TCC CCT CCC TGC CTC T
3' (nested)-TCT AGT CAT CCC CCA AGC TCC TC
CD154 5'-AGT CAG GCC GTT GCT AGT CAG T
3'-GGA AAC AAT GGA GAC TGC AGG TA
3' (nested)-TAA TGA GGA GTG GGC AGG CTC AG
TRANCE 5'-GAA AAC TTG CAG CTA AGG AGG GG
3'-GGT GGC CAA CAT CCT GCT TAT T
3' (nested)-CCT GCT TAT TAT TCA AGG CAT CC
CCR1 5'-GAT TTG GGC TCT TGG AAT CCT G
3'-GTG CTT AGC CCA CTC CCT GAA T
3' (nested)-AGG GCT TTC TTA GTT CCA CTG CC
CCR2 5'-TGG GAG TTT TGG TGG AGT CCG AT
3'-GGG GGA TGT GGC CTA AGA AGC AT
3' (nested)-GCC TAA GAA GCA TCT GAA CAA TGG
CCR3 5'-CTA AGG TCA TTA CCA CAG GCC AGG
3'-AGC AGG GAA AGA ACT AGG CAC ATT
5' (nested)-GCA GCG TAC TCA TCA TCA ACC C
CCR5 5'-CTC AGG GAA TGA AGG TGT CAG A
3'-TGC TAC TGT TGC ACT CTC CAC AAC T
5' (nested)-AGC CTC TGA ATA TGA ACG GTG AGC
CCR6 5'-CAT GGA ACT CAT GTT TTT AAA GGG C
3'-CCA TGC CTA GCC CAT GAC AGT A
3' (nested)-CCC ATG ACA GTA CCT TCC TAA CA
CCR7 5'-AGC ACA CTC ATC CCC TCA CTT G
3'-AGC CAA GAG CTG AGT GCA TGT C
3' (nested)-GAG TGC ATG TCA TCC CCA CTC T
CXCR1 5'-CAG ATC TAT GCC ACA AGA ACC CC
3'-CTT TCT AGG GAT GCT GAT GCT GC
3' (nested)-CTG ATG CTG CAC GCC AGC CTG GA
CXCR2 5'-ATT ACC AGG GAC TGA GGG GAG GG
3'-GTG GCA TTA AGT CAC ATT GCG G
3' (nested)-GTC ACA TTG CGG TAC AAC TAT CAC
CXCR3 5'-CAC TGC CCT TCT CAT TTG GAA ACT
3'-GCA AAT ATA GAG GTC TTG GGG AC
5' (nested)-AGT ACA AGG CAT GGC GTA GAG GG
CXCR4 5'-GGA CCT GTG GCC AAG TTC TTA GTT
3'-ACT GTA GGT GCT GAA ATC AAC CCA
3' (nested)-CAG CTG GGG ATC ATT TCT AGC TTT
CXCR5 5'-CAG GAC AAC GAG AAA GCC CTA AG
3'-GGT CTC TGT GCT GCC TGT ACT GT
5' (nested)-GTA TCT CCT CGC AAG CTG GGT AA
TCR Cβ 5'-TCA AGT CCA GTT CTA CGG GCT C
3'-TCA TAG AGG ATG GTG GCA GAC A
5' (nested)-CTC TCG GAG AAT GAC GAG TGG AC