. Author manuscript; available in PMC: 2007 Jan 25.

Published in final edited form as: J Proteome Res. 2005;4(6):2397–2403. doi: 10.1021/pr050160f

Table 3.

The quantities of recovered peptides^a and peptide/protein identifications^b from four single mouse brain voxels prepared by the TFE-based protocol and analyzed using LC-MS/MS with a linear ion trap mass spectrometer (LTQ).

	Recovered Peptide (μg)	Different peptides	Proteins
Voxel-3F5	23.0	2595	855
Voxel-3F7	15.5	1762	587
Voxel-3F8	15.5	2446	757
Voxel-3F9	25.0	1982	664

Peptides were resuspended in 50 μL of 50 mM NH₄HCO₃ and their concentrations were measured using BCA protein assay.

All peptides identified passed the filtering criteria discussed in detail in the experimental section.