Table 2.

Regulatory dependence of type III secreted effectors under SPI-2 inducing conditions.

		A	B	C	D	E	F	G	H	I
		wild type	MM + pWSK29	MM sprB+	MM hilC+	MM hilD+	MM hilA +	MM invF+sicA +	MM ssrB+	MM sirA +
1	sopA-lacZ	1221 ± 35	35 ± 13	24 ± 7	19 ± 7	26 ± 7	48 ± 17	6129 ± 1152	75 ± 36	33 ± 6
2	sopB-lacZ	3573 ± 34	99 ± 5	61 ± 7	61 ± 15	78 ± 10	44 ± 3	219791 ± 11811	33 ± 15	46 ± 5
3	sopE2-lacZ	3530 ± 270	5406 ± 547	4807 ± 538	7285 ± 1182	2347 ± 35	7141 ± 852	49681 ± 5581	7141 ± 2059	5222 ± 350
4	slrP-lacZ	1650 ± 24	1222 ± 92	3091 ± 43	1108 ± 132	1211 ± 163	1688 ± 523	1858 ± 238	1901 ± 311	2146 ± 34
5	sspH1-lacZ	3744 ± 140	1908 ± 254	1772 ± 471	1686 ± 161	1420 ± 116	1950 ± 128	3558 ± 232	3947 ± 978	1951 ± 154
6	sspH2-lacZ	6482 ± 1779	764 ± 123	1006 ± 48	655 ± 80	625 ± 84	981 ± 35	850 ± 102	62305 ± 14432	890 ± 111
7	sifA-lacZ	9995 ± 1326	13634 ± 1478	11691 ± 1186	10521 ± 1782	12603 ± 1877	13028 ± 2017	11010 ± 1742	8625 ± 784	21071 ± 1272
8	sifB-lacZ	2209 ± 503	1085 ± 77	711 ± 89	49 ± 3	1264 ± 139	1074 ± 207	43 ± 6	14677 ± 1092	43 ± 8
9	sseI-lacZ	3543 ± 314	119 ± 23	168 ± 40	130 ± 19	127 ± 32	128 ± 25	108 ± 16	11049 ± 2830	165 ± 10
10	sseG-lacZ	3017 ± 780	1444 ± 93	700 ± 49	1503 ± 109	1146 ± 118	690 ± 121	1083 ± 96	4229 ± 221	802 ± 99

The lac fusion being measured is listed in the left column. The strain background is listed along the top. Each strain harbors a lac fusion to an effector and a plasmid encoding a regulator or the vector control. The values in bold are those showing greater than 3-fold differences compared to the MM + pWSK29 strain that are discussed in the text. Each value represents the mean of at least three experiments ± standard deviation. MM, multi-regulator mutant [Δ (avrA-invH)1 ssrB::cat sirA4::hyg].