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Concentration- course changes of Ras, Rap1A, Akt, phospho-Akt, ERK1/2, phospho-ERK1/2 activation in NCI-H1819 cells treated with YM529 by using Western blot. Cells were incubated in CM with 10 % FBS. Equal amounts of protein were loaded in each lane. β-Actin was used as internal control.
