Figure 1.
The effect of porin on Toll-like receptor (TLR) and myeloid differentiation factor 88 (MyD88) mRNA expression. Lymphocytes of C57BL/6 mice were treated with and without 1 µg of porin per 1 × 105 cells for 72 hr. Total RNA was extracted from the sorted B-2 cells after 72 hr of culture and subjected to reverse transcription–polymerase chain reaction (RT–PCR). Ethidium bromide-stained PCR products were photographed, and then images were digitized and analysed. PCR products were quantified and expressed as the ratio of each product to glyceraldehyde-3-phosphate dehydrogenase (GAPDH) band density. The data shown are representative of three independent experiments.