Figure 4.

Effect of porin on nuclear factor-κB (NF-κB) mRNA expression. Lymphocytes were treated with or without porin for 72 hr. Total RNA was extracted from the sorted B-2 cells after 72 hr of culture and subjected to reverse transcription–polymerase chain reaction (RT–PCR). Ethidium bromide-stained PCR products were photographed and then images were digitized and analysed. PCR products were quantified and expressed as the ratio of each product to glyceraldehyde-3-phosphate dehydrogenase (GAPDH) band density. The data shown are representative of three independent experiments.