Table 3.
Summary of the antimycobacterial cellular and humoral immunological responses by Mycobacterium bovis-infected cattle (group 2 animals) in the week prior to PM (arranged by increasing PM score)
| Cellular assays† | Cytokineβ mRNA PCR | Antibody OD450 | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Animal | PM score | DTH* PPD-B Increase (mm) | LPA (net cpm) PPD-B | PWM | IFN-γ ODI | IFN-γ | IL-2 | IL-4 | IL-10 | IgG1 | IgG2 |
| B1 | 12 | 28·5 | 31 644 | 213 172 | 11·6 | + + + | + + | − | + | 0·09 | 0·16 |
| B5 | 17 | 11·5 | − | 189 585 | 12·4 | + + | + + | + − | + + + | 0·09 | 0·12 |
| B4 | 20 | 17 | 5240 | 2072 | 14·3 | + | + + | − | + + + | 0·37 | 0·28 |
| B2 | 22 | 15 | 2232 | 80 862 | 11·9 | + + + | + + | − | + + + | 0·38 | 0·16 |
| B6 | 25 | 12·5 | − | 49 493 | 4·9 | + + + | + + | − | + + | 0·76 | 0·77 |
| B3 | 47 | 8·5 | 999 | 3176 | 16·0 | + + | − | − | + + + | 0·87 | 0·16 |
*
Following collection of the last blood samples, animals were skin-tested. The DTH response was measured after 72 hr using callipers and reported as increase in skin thickness.
†
LPA and IFN-γ data are presented as follows. Net c.p.m. (test antigen c.p.m. − control c.p.m.), a net c.p.m. reported as negative = no net positive proliferation towards PPD-B. ELISA ODI (test antigen OD/control OD). All animals retained an IFN-γ response to PWM (ODI > 7).
‡
Cytokine mRNA PCR results are presented as scores relative to β-actin.