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LIGHT treatment causes induction of MMP-9 in macrophage enriched cells isolated from RA synovium. Adherent mononuclear cells isolated from synovial fluid of RA patients were stimulated with recombinant human LIGHT in the presence or absence of 100 U/ml IFN-γ. Culture supernatants were collected 24 h after activation and MMP-9 expression levels were measured using gelatin-zymogram. As a positive control, cells were treated with 1 µg/ml LPS.
