Figure 4.

Distribution/localization of APCs within the vaginal tract following IVAG CpG-ODN delivery. Four days following Depo-Provera treatment, groups of mice were either killed immediately or killed at 12, 28 or 48 hr following vaginal delivery of CpG-ODN. Immediately thereafter, vaginal tissues were collected, placed in OCT and frozen in liquid nitrogen. Immunohistochemical analysis of MHC class II, CD11b and F4/80 was performed on 8 µm cryostat sections using the immunoperoxidase technique. Images were captured using a 4× objective lens. Average sections, representative of at least three independent experiments using at least three mice/group are shown.