Figure 5.

Neither direct FcR cross-linking nor FcR saturation with human serum before sLAG-3 addition has any significant effect on CD1a expression. Monocytes were preincubated for 15 min at 37° with 1 μg/ml hIgG1 before the addition of goat anti-human immunoglobulin (10 μg/ml) to directly cross-link FcR (right) or for 30 min at 37° with 10% human serum to saturate FcR before sLAG-3 (1 μg/ml) addition (left). Cells were then cultured with GM-CSF and IL-4 for 6 days. At the indicated times, cells were removed and CD1a expression was assessed by FACS analysis. Data are given as the mean of mean fluorescence intensity (MFI) × percentage positive cells for three independent experiments.