Figure 1.
Effect of cross-linking CD11b on light scatter properties of normal human peripheral blood leucocytes. Dot-plots of forward scatter (cell size) versus side scatter (granularity) showing three main populations of leucocytes before (Control) and following in vitro cross-linking of CD11b (X-L CD11b). Lymphocytes were gated as region 1 (R1), monocytes gated as Region 2 (R2) and neutrophils as region 3 (R3). For the purposes of analysis, neutrophils were further subdivided into two main populations, i.e. P1, corresponding to size and granularity of control neutrophils and larger P2 cells which appear only following cross-linking of CD11b. The P2 gate was set according to the upper limit for forward scatter of control cells to ensure that all cells appearing in the P2 region were a direct result of cross-linking. In this example, cells were incubated at 37° for 30 min in cross-linking reagent: Goat F(ab′)2 anti-mouse IgG.