Figure 8.

Flow cytometric analysis of CD31 expression on untreated or on tumour necrosis factor-α (TNF-α)-treated monocyte-derived dendritic cells (moDCs). Cells were either untreated (solid line) or treated (dotted line) for 24 hr with TNF-α (100 U/ml) before staining with anti-CD31 monoclonal antibody (mAb) (clone 5H2). Controls consisted of untreated (filled histogram) or of TNF-α-treated moDCs (not shown owing to overlap) incubated with an isotype-matched irrelevant mAb. The data presented are representative of three independent experiments.