Semiquantitative mRNA measurements in synovial tissue of FcR γ-chain-/- and C57BL/6 (control) mice. Synovial mRNA levels of IL-1, IL-1Ra, MCP-1, and MIP-2, 6 and 24 h after induction of ICA. At certain points during the PCR reaction, samples were put on agarose gel and electrophoresis was performed. The number of the cycle in which the first band appeared was found: thus, a low cycle number means a higher mRNA content. Data were corrected for GAPDH signal. (Each group: n = 6.)