Figure 4.

Expression of FcγRs by resident peritoneal macrophages of C57BL/6 and DBA/1 mice as determined by fluorescence in FACS analysis. Almost twice as many receptors were expressed in DBA/1 as in C57BL/6 (control) mice. Cells were isolated from naïve mice using ice-cold medium and thereafter incubated with an anti-FcγR antibody (2.4G2) that recognises both FcγRII and RIII. Incubation with FITC-labeled secondary antibody followed.