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Phenotypic analysis of immature dendritic cells (iDCs) and mature DCs (mDCs) from interleukin (IL)-12/IL-23−/− and C57BL/6 mice. Bone marrow-derived DCs were cultured in the absence (iDCs) or presence (mDCs) of lipopolysaccharide (LPS) (1 µg/ml) for 18 hr. After washing, cells (106) were stained with fluorescence-labelled anti-CD11c, monoclonal anti-major histocompatibility complex (MHC) class II and monoclonal anti-CD86 (B7-2) immunoglobulins, and analysed by flow cytometry.
